The complexity of HLA-DS molecules. A homozygous cell line expresses multiple HLA-DS alpha chains
نویسندگان
چکیده
Ia molecules expressed by an HLA-DRw6 homozygous cell line were immunoprecipitated with anti-Ia allosera and monoclonal antibodies and analyzed by 2-D gel electrophoresis. The DRw6 homozygous cell line was shown to express two DS beta chains; this observation extends our previous finding that a DR5 homozygous cell line expresses two DS beta chains and suggests that the expression of at least two DS beta chains by DR homozygous cell lines is a generalized phenomenon. The data presented here document for the first time that a DR homozygous cell line expresses at least two DS alpha chains. Therefore, this cell line expresses at least two DS molecules with the potential for the expression of four DS molecules. In agreement with previous reports, the cell line was shown to express two DR beta chains and one DR alpha chain that combine to form two DR molecules. The molecular specificities of two MB1 allosera and two MB1 -like monoclonal antibodies were also compared in these studies. Both MB1 allosera isolated a single DS molecule, while the MB1 -like monoclonal antibodies isolated at least two DS molecules. Therefore, these studies document for the first time that anti-Ia reagents which are specific for the MB1 or MB1 -like determinants in population studies do not recognize the same Ia molecules in immunochemical studies. The data presented here for the expression of at least two DS alpha chains and the location of the MB1 allodeterminant on only one of multiple DS molecules are in agreement with recent studies at the gene level.
منابع مشابه
An HLA-DR5 homozygous cell line expresses two DS (I-A-like) molecules
Previous studies have indicated that LLA-DR homozygous cell lines express two DR molecules but only a single DS (I-A-like) molecule. This report demonstrates that an HLA-DR5 homozygous cell line expresses at least two distinct DS molecules. These two DS molecules are formed by the association of a single DS alpha chain with either of two DS beta chains. Four distinct Ia molecules have now been ...
متن کاملA Homozygous Cell Line Expresses Multiple HLA - DS Alpha Chains
The human Ia antigens are encoded within the HLA complex on chromosome six and are borne on molecules composed of two noncovalently associated glycoproteins o f 3 4 , 0 0 0 daltons (a chains) and ~28,000 daltons (/3 chains). Ia antigens are important in the effective collaboration between immunocompetent cells in the generation of immune responses; they have also been associated with putative i...
متن کاملLine Expresses Multiple HLA - DS Alpha Chains
The human Ia antigens are encoded within the HLA complex on chromosome six and are borne on molecules composed of two noncovalently associated glycoproteins o f 3 4 , 0 0 0 daltons (a chains) and ~28,000 daltons (/3 chains). Ia antigens are important in the effective collaboration between immunocompetent cells in the generation of immune responses; they have also been associated with putative i...
متن کاملStructural analysis of a human I-A homologue using a monoclonal antibody that recognizes an MB3-like specificity
Monoclonal antibody IVD12 was used to isolate and characterize a human Ia molecule present on B cells that generally display DR4 or DR5 phenotypes. The specificity of binding of IVD12 to human peripheral blood B cells from 75 normal individuals and 19 homozygous human lymphoblastoid B cell lines was identical to the supertypic specificity MB3 previously defined. Furthermore, IVD12-reactivity wa...
متن کاملThe HLA-DR4 family of haplotypes consists of series of distinct DR and DS molecules
Among DR4-associated HLA-D antigens, distinct and consistent structural variations were found for the products of two human "Ia-like" loci, DR and DS. Analysis of neuraminidase-treated immunoprecipitated DR molecules from 15 HLA-DR4-associated HLA-D homozygous B-lymphoblastoid cell lines by two dimensional polyacrylamide gel electrophoresis identified five distinct DR beta chains. In addition, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 159 شماره
صفحات -
تاریخ انتشار 1984